What causes vaginal lubrication? Arousal, lubrication AND the vestibular glands. The greater and lesser vestibular glands or bartholin and skene glands secrete fluid which provides lubrication. However, this is not the primary source of vaginal lubrication. Vaginal lubrication Wikipedia T Shirt Vagina: Anatomy, Function & What to Look Out For Primarily caused by declining estrogen levels, it can result in discomfort, pain during intercourse, and an increased risk of vaginal infections. Less estrogen results in lower blood flow and lubrication, causing thinning The Bartholin's gland is located at the entrance to the vagina, and it secretes mucus, providing vulval and vaginal lubrication. A Bartholin's cyst occurs when the gland becomes blocked, and this can